The Prevalence of CYP2B6 Gene Polymorphisms in Malaria-endemic Population of Timor in East Nusa Tenggara Indonesia

10/15/2019 12:00:00 AM

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Authors:Linawati Hananta, Indwiani Astuti, Ahmad Hamim Sadewa, Josephine Alice, Jontari Hutagalung, Mustofa

Publication date: 23 July 2018

Osong Public Health and Research Perspectives

Volume:  9(4):192-196

Link: http://www.kcdcphrp.org/journal/view.html?doi=10.24171/j.phrp.2018.9.4.08

 

 

ABSTRACT

 

Objectives: The  CYP2B6 is one of the most polymorphic CYP genes in  humans that has  the potential to modify the pharmacological and toxicological responses to clinically important drugs such as antimalarial artemisinin and its  derivatives. The  aim  of the study was to determine the frequency of  CYP2B6 polymorphisms in  Timor malaria  endemic  area, East Nusa Tenggara, Indonesia where Artemisin-based Combination Therapy (ACT) has  been used to treat uncomplicated malaria.

Methods :  A total of  109  healthy subjects were participate d  in  this study.  CYP2B6*4,  *6 and *9 polymorphisms were analyzed using PCR-RFLP to confirm the SNPs prevalence of 516G>T  and 785A>G in exon 4 and 5

 

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Results:Therewere96subjectsincludedintheanalysis.Intheexon4ofCYP2B6516G>T,  thefrequency oftheTmutationwas37.5%(39/96),andthewildtype27.1%(26/96).Intheexon5,CYP2B6785A>G mutantwasdetectedin  29.2%(28/96)ofindividuals,andthewildtypeallelein  35.4%(34/96).The frequencyofCYP2B6*9(516G>T), CYP2B6*4(785A>G)andCYP2B6*6(516G>Tand785A>G)were40.6%, 29.2%and 22.9%,respectively.Theprevalenceofthese CYP2B6genepolymorphismsinTimorianethnic werehigherthanthatinMalay,Han  Chinese,Indian,andEgyptianpopulations.

Conclusion:The  prevalenceoftheseCYP2B6516G>T  and785A>G  polymorphismsinTimorianethnicis higherthanthatinother populations.Thesepolymorphismsmay affectthemetabolismofartemisinin anditsderivatives.