ANALYSIS OF PERIODIC SUBCULTURE AND WITH STERILE WATER METHODS FOR Aspergillus sp. AND Candida albicans
11/4/2020 12:00:00 AM
ABSTRACT
Introduction: A good and proper preservation can maintain all collection of culture. Periodic subculture
preservation is an old technique that is less able to guarantee the genetic characteristic for a long time. Sterile
water is an alternative method in various studies to ensure the genetic characteristic for a long time and can
be done in a simple laboratory concept. This study aimed to examine the method of periodic subculture and
with sterile water
Methods: This research was a descriptive study with an experimental laboratory design on Aspergillus sp.
and Candida albicans. This study examined at periodic subculture methods and with sterile water in
maintaining viability, level of contamination, morphology, and antifungal resistance after six months
Results: The fungus had lived after being preserved with periodic subcultures without contamination,
morphological changes, and resistance. Aspergillus sp. and Candida albicans also lived after being preserved
with sterile water for six months with the same morphology and without contamination. The zone of inhibition
of Aspergillus sp. had decreased but in Candida albicans remains the same.
Conclusion: Preservation with periodic subculture and with sterile water can be applied to Aspergillus sp. and
Candida albicans.
Key Words: Aspergillus sp., Candida albicans, periodic subculture, sterile water
