Essential Oil Composition, In Vivo Antioxidant, and Antimicrobial Activi- ties of Pimpinella pruatjan from West Java, Indonesia

4/4/2018 12:00:00 AM

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Authors:Agustina D.R. Nurcahyanti, Issam J. Nasser, Frank Sporer, Bernhard Wetterauer, Ireng D. Kadarso, Jürgen Reichling and Michael Wink

Publication date: March 2018

The Natural Products Journal

Volume:  Vol 8, Number 1, 61-69

Link: https://www.eurekaselect.com/153444/article

 

 

Background: The root of the Indonesian Pimpinella pruatjan Molk. has been mainly used as an aphrodisiac  and for the treatment  of Erectile Dysfunction  (ED). Since oxidative  stress favors ED, we examined the antioxidant activities of the essential oil from the root of P. pruatjan.

Methods: The chemical composition  of the essential oil was analyzed by Gas Liquid Chromatogra-phy-Mass  Spectrometry  (GLC-MS).  In vivo  antioxidant  activity  was  determined  using  an  animal model, the nematode Caenorhabditis  elegans, whereas the antimicrobial  activity of the essential oil was investigated using standard micro-dilution methods.

Results:  The  main  components  of  essential  oil  were  thymol  methyl  ether  and  2,5-dimethoxy-p- cymene; another major compound was tentatively identified as 2-isopropenyl-5-methyl-benzene-1,4- diol. Root essential  oil showed  substantial  in vivo antioxidant  activities,  demonstrated  by reduced hsp-16.2p::GFP  expression  in the pharynx  of C. elegans  and increased  survival  of worms  under acute stress conditions.  Antimicrobial  activity  showed  moderate  activity  against  bacteria  and Can- dida yeasts.

Conclusion: The antioxidant property shown in these findings needs further investigation,  especially with the major active compounds.

 

Keywords: Pimpinella pruatjan Molk., Apiaceae, essential oil, antioxidant,

Caenorhabditis elegans, antimicrobial.